2. Materials and methods
2.1. Materials
The pistachio nut oil cake was prepared from Pistacia vera L.
varieties at the oil extraction factory in Manila, Kerman, Iran. The
globulin fraction was extracted as described by Shokraii and Esen
(1988) and final globulin purity was measured at 80%. Tween-80
(emulsifier), calcium nitrate, glycerol (83% purity), and pure palmitic
(C16) and stearic (C18) acids were purchased from Merck
(Germany). Anhydrous calcium chloride and potassium sulfate
were obtained from Fluka (France).