The bags with banana puree were wrapped with an outer polyethylene bag containing water, heat sealed, and exposed to HHP treatments of 517 or 689 MPa for 10 min (holding time) at 21°C. The HHP treatments were applied in a warm isostatic pressing system (Engineered Pressure Systems, Inc., Andover, MA) with a cylindrical pressure chamber (height = 0.25m, diameter =0.10m). A 5% Mobil Hydrasol 78 water solution was used as the pressure medium. The pressure build-up velocity was 2.4 MPa/s after an initial delay of 45s. The come-up time required to reach 517 and 689 MPa was 4.4or 5.6 min, respectively. The decompression time was <15s. The slow pressure build-up velocity obtained in our work assured minimum adiabatic heating (De Cordt et al., 1995) and temperature variations were limited to within a few °C.Unblanched (control), blanched, and blanched-HHP treated banana purees were stored at 25±0.5°C for 15 days for color and microbiological analyses. Survival of microorganisms and color of the banana purees were determined daily during storage.
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