The Mw/Mn ratios of GP-LRS3 and
ZP-LRS3 were close to 1; this confirmed that LRS was more likely
to form a stable double helix structure. The polarization cross disappeared
and granules became irregular and showed a rough surface
compared with native starch and HAMS. Lotus seed resistant
starch showed B-type crystal structure and an increase in molecular
order than any of the other starch types analysed. Lotus seed
resistant starch is a physically modified starch. Analysis using
NMR indicated an increase of the double helix and higher crystallinity
in the LRS relative to the other starch samples analysed.
Moreover, the results also failed to demonstrate any difference
between purified resistant starches obtained by the different
purification methods.
In addition, LRS can increase the proliferation of bifidobacteria
compared with growth in the presence of GLU or HAMS. This effect
may be related to its stable double helix structure, high crystallinity,
and rough surfaces with a scale-like or gully-like appearance.