The results for chymosin show that salting-in behavior is observed
in NaCl solutions within the pH range of 4.0 to 5.0. Disruption of
attractive electrostatic configurations by protonation of acidic
residues (pH 3.0) or increased negative charge (pH 6.0) is a likely
cause for this change in low-salt behavior. The concentration
dependence of the amorphous precipitates within the salting-in
region, in conjunction with the apparent irreversibility of formation of
both amorphous aggregate morphologies in their mother liquor,
indicates that there may be a kinetic