3. Results and discussion The antimicrobial activities of soil, saliva and mixture of both soil and saliva were tested against the three tested pathogenic organisms. The results revealed that, soil alone showed moderate antimicrobial activity compared to saliva which did not produce any antagonistic effect. On the other hand, mixture of soil and saliva showed the highest antimicrobial activity (Fig. 1). The same trend was observed for the cell free bacterial and fungal culture media. Thus, the pathogenic organisms showed higher sensitivity to the filtrates resulted from mixing soil and saliva. Based on the above mentioned results, all the experiments were carried out using the mixture of soil and saliva. This is may be due to that the alkalinity of saliva may favour the growth of antibiotic producing microorganism. Furthermore, saliva act as oral antiseptic because it contain