We used a DNA encoding 30 amino acid residues corresponding to peptide [Ser8, Gln26, Asp34]-GLP-1 (7-36). The GLP-1 analogue gene was inserted into the variable region of the rice 26 kDa globulin gene between residues 109 and 110 (Fig. 1A) [18]. Furthermore, two junction sites of GLP-1 analogue and globulin were replaced by lysine and arginine residues, respectively, for trypsin