2.5. Surface conditioning
Based on previous work (Nitschke et al., 2009) we select three
concentrations of each biosurfactant for the pre-conditioning ofpolystyrene surface: rhamnolipids 0.25%, 0.5% and 1.0% (w/v)
aqueous solutions and surfactin 0.1%, 0.25% and 0.5% (w/v) aqueous
solutions. A 96 well polystyrene microplatewas filled with 200 ml of
each concentration of biosurfactant. After 24 h at room temperature,
solution was removed and the wells were gently washed 3
times with 200 ml of sterile distilled water. Control wells were filled
with 200 ml of water.