3. Results and discussion
3.1. Comprehensive evaluation of the biolipid-accumulating ability of strains in the yeast collection
The screening flow scheme is shown in Fig. 1a. Thirteen strains with high lipid productivity were obtained through primary and secondary screening. During primary screening, a comprehensive evaluation of the lipid-accumulating ability of yeast strains (a total of 500 strains) was carried out. In this experiment, SD medium was employed, as almost all strains could grow in this medium. The distribution of the lipid accumulation level is indicated in Fig. 1b. Approximately 6% of the tested strains (total 31 strains) accumulated total lipids to more than 0.8 g/L of the medium volume. The yeast strains examined in this study are listed in Table 1. As expected, the yeast strains which accumulated more than 0.8 g/L belonged to the basidiomycetes (for details, see below) and were used for further analysis.