Stock solutions were prepared at 10 mM in 5% DMSO: 95% PBS buffer. The
stock solutions were diluted to decreasing molarity across the plate with 5%
DMSO: 95% PBS buffer. Each plate was read vertically, with a gain of 30 and a
laser intensity of 90% at 635 nm to produce raw data of counts per well. All raw
data was processed using the BMG LABTECH NEPHELOstar Galaxy Evaluation
software. The solubility limit was determined by the significant increase in the
signal/noise ratio.