3.1.2. Effect of CPMS concentration and temperature on derivatization
S. Zhang et al. / Talanta 98 (2012) 95–100 97
The effects of CPMS concentrations on derivatization were studied in detail to ensure sufficient reaction of the analytes. The results indicated that constant fluorescence intensity was achieved with the addition of a six-fold molar reagent excess to total molar fatty acids. Further increasing the excess of reagent beyond this level had no significant effect on the yields. The effect of the reaction temperature on derivatization was also evaluated. The result indicated that a lower reaction temperature needed a longer reaction time.