3.3. Identification of bacteria TB09 and TB072
The two bacterial antagonistic strains TB09 and TB72 were
identified by morphological observation, physiological test and
DNA sequence analysis. The results showed that TB09 was most
similar to Bacillus pumilus strain AY876289 (98.01%); similarity
overlap included Bacillus altitudinis strain AJ831842 (96.22%) as
second comparison and Bacillus stratosphericus strain AJ831841
(94.58%) as third comparison. Based on colony appearance criteria,
the identity of TB09 was best assigned as a B. pumilus strain. Likewise,
the results obtained from sequence comparison indicated
TB72 was most similar to Bacillus thuringiensis strain D16281
(99.50%) and, to a lesser extent, Bacillus cereus strain HE716871
(85.63%). Thus TB72 was identified as a B. thuringiensis strain.