In denitrification by resting cells, KN03 (1.08 g/l) or KNOz (1.0 g/l) was used as the terminal electron acceptor for denitrification (see Results and Discussion).
Denitrifying growth experiment A 300-ml conical flask (200 ml of denitrifying medium) with a silicon stopper was used in the experiment. After the addition of 0 (control), 2 or 5% PVA and 3 or 5 g/l of boric acid to the medium, the flasks were autoclaved (lOlb, lOmin), then the preculture broth was added (lo%, v/v). The flasks were incubated for 3 d at 30°C under dark anaerobic conditions after sparging with pure nitrogen gas. Flasks were shaken by hand at 12-h intervals to maintain homogeneity.
In denitrification by resting cells, KN03 (1.08 g/l) or KNOz (1.0 g/l) was used as the terminal electron acceptor for denitrification (see Results and Discussion).Denitrifying growth experiment A 300-ml conical flask (200 ml of denitrifying medium) with a silicon stopper was used in the experiment. After the addition of 0 (control), 2 or 5% PVA and 3 or 5 g/l of boric acid to the medium, the flasks were autoclaved (lOlb, lOmin), then the preculture broth was added (lo%, v/v). The flasks were incubated for 3 d at 30°C under dark anaerobic conditions after sparging with pure nitrogen gas. Flasks were shaken by hand at 12-h intervals to maintain homogeneity.
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