The Alamar Blue assay has been exploited for monitoring immune cell proliferation and function.
Immune cells including lymphocytes, monocytic macrophage cell lines, interleukin-dependent
cytotoxic T cell lines, dendritic cells and myeloma cells have been monitored by Alamar Blue
assays , since the assay does not require cell lysis and continuous monitoring through time-course experiments is possible [64,65]. It was also used in time-course experiments to investigate immuno-modulatory effects . The specific advantages of the Alamar Blue assay over the [3H]thymidine assay for lymphocyte proliferation studies are: (i) non-radioactive, (ii) ease of use, (iii) lower cost, (iv) not labor intensive, (v) rapid assessment of proliferation of large number of samples; (vi) non-toxic; (vii) useful in determining the kinetics of cell growth of hybridomas, and (viii) no interference between secreted of antibodies of a given hybridoma cell line and assay chemistry
The Alamar Blue assay has been exploited for monitoring immune cell proliferation and function.Immune cells including lymphocytes, monocytic macrophage cell lines, interleukin-dependentcytotoxic T cell lines, dendritic cells and myeloma cells have been monitored by Alamar Blueassays , since the assay does not require cell lysis and continuous monitoring through time-course experiments is possible [64,65]. It was also used in time-course experiments to investigate immuno-modulatory effects . The specific advantages of the Alamar Blue assay over the [3H]thymidine assay for lymphocyte proliferation studies are: (i) non-radioactive, (ii) ease of use, (iii) lower cost, (iv) not labor intensive, (v) rapid assessment of proliferation of large number of samples; (vi) non-toxic; (vii) useful in determining the kinetics of cell growth of hybridomas, and (viii) no interference between secreted of antibodies of a given hybridoma cell line and assay chemistry
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