Optimized conditions
For β-glucan production in industrial scale, every step of the extraction method has to be optimized. Optimized conditions, especially those concerning the critical steps for glucan preparation (cell lysis, NaOH, enzymes) are mentioned in Methods 1, 2, and 3.[21-23] In the other methods, the critical step process conditions are not referred. The optimization of each extraction step is reviewed in Table 2.