-Limulus lysate for endotoxin
*Gram- bacteria produce endotoxin, which consists of lipopolysaccharide (LPD)
*Limulus amoebocyte lasate (LAL) obtain from the blood cell of the horseshos crab
*LAL is sensitive to endotoxin
*adding of food aliquots of food suspension to a lysate portion
*incubation, endotoxin causes the gel formation