The amplifcation conditions were performed in a 50 µL reaction volume as follows: 1 × PCR buffer (Invitrogen™ Life Technologies, Foster, CA), 0.2 mM each dNTP, 0.3 µM of each primer, 1.5 mM MgCl2, 0.8 units Tag DNA Polymerase (Invitrogen™ Life Technologies), and 100 ng DNA