Conclusion
In this study. The simultaneous derivatization/DLLME was established for the preconcentration of amandine in biological fluids followed by GC-FID determination. The method shows relatively low matrices effects in the completely complex samples.The developed method provided several advantages including simplicity, less solvent and time-consuming, low detection limits and high EF and EnF for the determination of amantadine at ng mL-1 levels in the human urine and plasma samples.