Three commercial rice flours (indica, japonica and glutinous) were applied to prepare the whole rice cake in chiffon style in comparison to that made of low gluten wheat flour. Not only the in vitro and in vivo digestion investigations but also the physicochemical properties were discussed. The contents of rapidly digestible starch in rice cakes were ranged in 38%–45%, and the contents of resistant starch were complementary to locate in 55–62% since the slowly digestible starch was very limited. The in vitro tests based on white bread as a reference food resulted rice cakes in low-to-middle GI foods by Granfeldt and Goni models, while the in vivo test based on the glucose as a reference food showed the rice cakes and wheat cake satisfied the low GI criteria. Transferred Goni model described a good consistency to the in vivo result. Specific volume of indica rice cake was resembling to that of wheat cake, and they were both higher than the other two rice cakes. Color measurement of all cakes performed similar to one another. Texture test revealed that the fresh indica cake had lowest hardness and chewiness as well as higher resilience and springiness than the other rice samples. After 24-h storage, the hardness and chewiness of indica cake turned to higher than the other rice cakes due to the fast aging.