The content of chlorophylls can be determined photometrically following extraction of the pigments using an
organic solvent, such as acetone or dimethyl formamide, or else by means of handheld device based on lightemitting
diodes and silicon photodiode receptor that measures leaf transmittance in the red (650 nm) and infrared
(950 nm) regions of the electromagnetic spectrum5. The transmittance values are used by the device to derive a
relative SPAD meter values that is proportional to the amount of chlorophyll in the sample. The former method is
considered as in vitro measurement which is well established and accurate, but time-consuming, destructive, and
requires the use of toxic or flammable chemicals. On the other hand, the latter provides an alternative in vivo
method for the measurements of relative leaf chlorophyll levels that overcome these disadvantages, but it is less
accurate, not applicable for small or thick leaf, influenced by light condition, and produces only predictive value.
The content of chlorophylls can be determined photometrically following extraction of the pigments using anorganic solvent, such as acetone or dimethyl formamide, or else by means of handheld device based on lightemittingdiodes and silicon photodiode receptor that measures leaf transmittance in the red (650 nm) and infrared(950 nm) regions of the electromagnetic spectrum5. The transmittance values are used by the device to derive arelative SPAD meter values that is proportional to the amount of chlorophyll in the sample. The former method isconsidered as in vitro measurement which is well established and accurate, but time-consuming, destructive, andrequires the use of toxic or flammable chemicals. On the other hand, the latter provides an alternative in vivomethod for the measurements of relative leaf chlorophyll levels that overcome these disadvantages, but it is lessaccurate, not applicable for small or thick leaf, influenced by light condition, and produces only predictive value.
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