Mixed
culture
of
Saccharomyces
cerevisiae
and
Acetobacter
pasteurianus
was
carried
out
for
high
yield
of
acetic
acid.
Acetic
acid
production
process
was
divided
into
three
stages.
The
first
stage
was
the
growth
of
S.
cerevisiae
and
ethanol
production,
fermentation
temperature
and
aeration
rate
were
controlled
at
32◦C
and
0.2
vvm,
respectively.
The
second
stage
was
the
co-culture
of
S.
cerevisiae
and
A.
pasteurianus,
fermentation
temperature
and
aeration
rate
were
maintained
at
34◦C
and
0.4
vvm,
respectively.
The
third
stage
was
the
growth
of
A.
pasteurianus
and
production
of
acetic
acid,
fermentation
temperature
and
aeration
rate
were
controlled
at
32◦C
and
0.2
vvm,
respectively.
Inoculation
volume
of
A.
pasteurianus
and
S.
cerevisiae
was
16%
and
0.06%,
respectively.
The
average
acetic
acid
concentration
was
52.51
g/L
under
these
optimum
conditions.
To
enhance
acetic
acid
production,
a
glucose
feeding
strategy
was
subsequently
employed.
When
initial
glucose
concentration
was
90
g/L
and
120
g/L
glucose
was
fed
twice
during
fermentation,
acetic
acid
concentration
reached
66.0
g/L.
Mixed
culture
of
Saccharomyces
cerevisiae
and
Acetobacter
pasteurianus
was
carried
out
for
high
yield
of
acetic
acid.
Acetic
acid
production
process
was
divided
into
three
stages.
The
first
stage
was
the
growth
of
S.
cerevisiae
and
ethanol
production,
fermentation
temperature
and
aeration
rate
were
controlled
at
32◦C
and
0.2
vvm,
respectively.
The
second
stage
was
the
co-culture
of
S.
cerevisiae
and
A.
pasteurianus,
fermentation
temperature
and
aeration
rate
were
maintained
at
34◦C
and
0.4
vvm,
respectively.
The
third
stage
was
the
growth
of
A.
pasteurianus
and
production
of
acetic
acid,
fermentation
temperature
and
aeration
rate
were
controlled
at
32◦C
and
0.2
vvm,
respectively.
Inoculation
volume
of
A.
pasteurianus
and
S.
cerevisiae
was
16%
and
0.06%,
respectively.
The
average
acetic
acid
concentration
was
52.51
g/L
under
these
optimum
conditions.
To
enhance
acetic
acid
production,
a
glucose
feeding
strategy
was
subsequently
employed.
When
initial
glucose
concentration
was
90
g/L
and
120
g/L
glucose
was
fed
twice
during
fermentation,
acetic
acid
concentration
reached
66.0
g/L.
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