The experiment was conducted at Mushroom Ambra Biotech Sdn Bhd, Kulai Jaya, Johor Bahru, Malaysia. Pure culture of Pleurotus spp. was prepared on agar medium composed of 20 g agar and 10 g dextrose. The mixture was boiled on a gas burner until it was solubilized. The medium was dispensed into glass Petri dishes (100 × 15 mm). The Petri dish containing the medium was sterilized in an autoclave for 20 min at 120°C under 1 kg/cm2 pressure. The solidified media were inoculated with Pleurotus spp. The tissues were collected from the inner region of stalk of sporophore. The
tissues were cut into pieces and placed on the solidified Petri dish containing potatoes dextrose agar. The inocula were transferred to a growth chamber which was maintained at ≤ 30°C and incubated for eight days.