The studies of AChEs activity were carried out with a SHIMADZU UV-1800 spectrophotometer. Enzyme kinetics were measured using Ellman’s method which is based on the reaction between the
reaction product thiocholine and 5,50-dithiobis(2-nitrobenzoic acid) (DTNB), leading to a yellow compound (5-thio-2- nitrobenzoate) absorbing at 412 nm (e = 13,600 M1 cm1).