QUALITATIVE ( SCREENING ) PROCEDURE
Each test requires 4 wells of a microtitre plate.
1. Dispense Diluent into the microtitration plate as follows:
25l in rows 1, 3 & 4 and 100l in row 2.
2. Dispense 25l of each sample into a well in row 1.
Mix well and transfer 25l from row 1 to row 2.
Mix well and transfer 25l from row 2 to row 3.
Mix well and discard 25l from row 3.
Transfer 25l from row 2 to row 4.
Mix well and discard 25l from row 4
3. Add 75l of well mixed Control Cells to row 3.
4. Add 75l of well mixed Test Cells to row 4.
Tap plate gently to mix.
The final dilutions in row 3 and 4 are 1/80.
5. Cover and let stand at room temperature for 45 to 60 minutes (alternatively the plates can be left overnight).
6. Examine for agglutination patterns.
Note: Kit controls are prediluted and should be added directly into individual wells in row 3 and 4 ( no diluent required ).