In indirect ELISA, all four TuMV isolates,
YC5, RC4, L, and R, reacted positively
with TuMV but weakly with KMV
and DsMV antisera, including one purchased
from Agdia (Table 1). In direct
ELISA, they reacted strongly with TuMV
antiserum but not with KMV or DsMV
antiserum. In reciprocal tests, KMV and
DsMV antigens did not react with TuMV
antisera in both indirect and direct ELISA
(Table 1). Prepared against bacterium-expressed
coat protein (CP) antigen, the
KMV antiserum reacted with its homologous
antigen only in indirect but not in
direct ELISA (Table 1). Unlike TuMV,
KMV showed no serological relationship
with DsMV in indirect or direct ELISA
(Table 1).
In indirect ELISA, all four TuMV isolates,YC5, RC4, L, and R, reacted positivelywith TuMV but weakly with KMVand DsMV antisera, including one purchasedfrom Agdia (Table 1). In directELISA, they reacted strongly with TuMVantiserum but not with KMV or DsMVantiserum. In reciprocal tests, KMV andDsMV antigens did not react with TuMVantisera in both indirect and direct ELISA(Table 1). Prepared against bacterium-expressedcoat protein (CP) antigen, theKMV antiserum reacted with its homologousantigen only in indirect but not indirect ELISA (Table 1). Unlike TuMV,KMV showed no serological relationshipwith DsMV in indirect or direct ELISA(Table 1).
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