2.8. Statistical analysis
Two independent replicates were measured at least two times in
each experiment and the results were reported as the mean and
standard deviation. The statistical analysis was performed by analysis
of variance (ANOVA) and test of least significant difference (LSD)
using the statistical program Statgraphics Plus v5.1 (Statgraphics
Corporation; USA, 2000). Significance was considered at p < 0.05.