There are currently attempting to supply renewable energy efficiency to replace fossil fuels. This research aims to study the bio-gas from pig manure used in motor tricycle. The application of a 356 CC engine in Daihatsu motor tricycle. Equipped with 25 liters of LPG cylinder. The filling gas into the tank by using a combination of bio-gas, packaging size 1.5 horsepower electric motor, containing gas pressure of 180 pounds per square inch. It takes an average of 5.75 minutes after filling gas to make driving on the road to test for effectiveness and found a speed of 19 kilometers per hour. Motor tricycle can run at an average distance of 1.18 kilometers.