The almost-complete 16S rRNA gene sequence (1419 nt) of strain SR14.14T was compared against related sequences in the GenBank database. The results indicated that this isolate belongs to the genus Sphaerisporangium. The strain SR14.14T showed the highest level of 16S rRNA gene similarity with the type strain of S. album (97.8%, a value corresponding to 29 nucleotide differences from 1328 locations). The 16S rRNA gene sequence similarity value of strain SR14.14T with the type strains of S. melleum, S. viridialbum and S. cinnabarinum were 97.8, 97.6 and 97.2%, respectively.
It is evident from the phylogenetic tree with members of the genus Sphaerisporangium and type strains representing species of the family Streptosporangiaceae that strain SR14.14T is closely related to S. album, with a bootstrap value of 75% by neighbor-joining analysis (Figure 2). Similar tree topographies were obtained when the least-squares and maximum-parsimony methods were applied. The isolate showed low bootstrap values with other members of the genus Sphaerisporangium. DNA–DNA relatedness studies were carried out between strain SR14.14T and S. album DSM 45172T by reciprocal hybridizations. The DNA–DNA reassociation values were in the range of 32–39%, which confirmed that the strain SR14.14T represented a separate genomic species based on whole-genomic DNA relatedness of less than the 70% cut-off point.29
The almost-complete 16S rRNA gene sequence (1419 nt) of strain SR14.14T was compared against related sequences in the GenBank database. The results indicated that this isolate belongs to the genus Sphaerisporangium. The strain SR14.14T showed the highest level of 16S rRNA gene similarity with the type strain of S. album (97.8%, a value corresponding to 29 nucleotide differences from 1328 locations). The 16S rRNA gene sequence similarity value of strain SR14.14T with the type strains of S. melleum, S. viridialbum and S. cinnabarinum were 97.8, 97.6 and 97.2%, respectively.It is evident from the phylogenetic tree with members of the genus Sphaerisporangium and type strains representing species of the family Streptosporangiaceae that strain SR14.14T is closely related to S. album, with a bootstrap value of 75% by neighbor-joining analysis (Figure 2). Similar tree topographies were obtained when the least-squares and maximum-parsimony methods were applied. The isolate showed low bootstrap values with other members of the genus Sphaerisporangium. DNA–DNA relatedness studies were carried out between strain SR14.14T and S. album DSM 45172T by reciprocal hybridizations. The DNA–DNA reassociation values were in the range of 32–39%, which confirmed that the strain SR14.14T represented a separate genomic species based on whole-genomic DNA relatedness of less than the 70% cut-off point.29
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