The vegetative and reproductive tissues viz., shoot and seed explants showed varied responses in different combinations of media (Table 1). Among the auxins tried, NAA supplemented at 2.68µM and 5.37µM induced callus from shoot explants whereas no callus was produced in IBA supplemented media, which induced rooting. The callus was hard, organogenic and white in colour initially which proliferated and turned green in colour on transfer to the same medium and small shoot buds initiated after 45 to 60 days in culture. In media supplemented with cytokinins, BA and kinetin, at 2.22µM and 4.44µM, singly, only multiple shoots were produced and no callus induction was noticed. When auxins and cytokinins were tried in combination, Interaction of 2.68µM NAA and 4.44µM BA gave the most favourable response. In this medium callus was induced at the base of the shoot explants and in root tip explants (Fig. 1a) after 30days of incubation. Further subculture into MS media with 4.44µM BA and 2.68µM NAA, regeneration of shoot buds occurred from these calli (Fig. 1b). About 5-20 shoot primordia were formed from each mass and each of them was capable of regenerating into individual shoot and plantlet, which were studied in comparison with parent (control), Vanilla planifolia.