Blood and tissue sample processing
After 12 weeks of the experimental period, the rats were
sacrificed following 16 h of starvation, by decapitation. The liver,
heart, epididymal, and retroperitoneal fat pads were removed
quickly, weighed, and stored at -70℃ until biochemical analysis.
Blood samples were collected and centrifuged at 3,000 rpm at
4℃ for 15 min, and the supernatants were stored at -70℃ for
biochemical analysis.