To 0.5 mL diluted organic extract, 0.5 mL of 2% alumi-num chloride (AlCl3) dissolved in methanol was added . The sample was
incubated for 15 min at room temperature and the absorbance of the
reaction mixtures was measured at 430 nm. Rutin was used as standard
flavonoid. The total flavonoid content was expressed as mg of rutin
equivalents per 100 g DW, by using a standard graph and the values
were presented as means of triplicate analyses.