Conclusions
In this study, optimal liquefaction and saccharification combinations of three important growth parameters, temperature, enzyme dose, dry weight for liquefaction and temperature-enzyme dose, and time for saccharification were generated using RSM with Box–Behnken design. Optimum combinations were 95 °C, 1 mL of α-amylase, and 4.04 g dry weight of waste potato mash per 100 mL of deionized water and 60 °C, 0.8 mL of amyloglucosidase, and 72 h for liquefaction and saccharification, respectively.