Healthy Japanese individuals participating in the study followed proposed dietary schemes without significantly loosing or gaining body weight (mean [±SD], 58.7 ± 9.3 at the start of the trial, 58.9 ± 9.1 kg after the non-Japanese and 58.7 ± 9.3 after the Japanese diet). The trial subjects did not express any inconvenience following the Japanese diet; on the contrary, it was associated with an old-fashioned Japanese diet consumed at participants’ parents/grandparents homes. At the same time it was difficult to follow the control diet. Therefore, a dietary expertise was used to adjust the control diet and to ensure its nitrate levels to be within the ADI range. Individually consumed daily nitrate intake was approximated to a mean concentration of 18.8 mg/kg of body weight/day during the Japanese diet study phase. Nitrate, naturally derived from Japanese diet exceeded five times the Acceptable Daily Intake (ADI = 3.7 mg/kg/body weight).