Feathers, skin, striated muscle, uropygial gland, heart-liver (combined), and
stomach with contents were separated from
individual hooded pitohuis, variable pitohuis (P. kirhocephalus), and rusty pitohuis
(P. ferrugineus) (2). Each tissue was stored
separately in 100% ethanol and later was
macerated and washed with 100% ethanol.
These crude ethanol extracts were concentrated so that 100 k1 of extract were equivalent to 100 mg of tissue.