The factors that might influence the extraction were investigated,
such as time, temperature, the ratio of tri-n-oxtylamine (carrier) to
oleyl alcohol (solvent) on the base of volume (RCS), the ratio of
solvent phase to fermentation on the base of volume (RSF), the concentration
of lactic acid in aqueous solution and pH values (Table 1).
The ratio of solvent phase (with lactic acid) to water (~90 oC) on the
base of volume (RSW) was investigated for the back extraction.