he variety of the olive fruit or the maturity stage.
Up to date, SDS-PAGE is the most used technique to separate
olive proteins and to determine their molecular masses
[1 ,4,5,9–13]. Further refinement of the technique has been
recently observed by the off-line analysis of the SDS-PAGE bands
using MALDI-TOF MS and/or nanoLC-MS/MS [14,15]. Capillary
electrophoresis (CE) presents a high potential for the separation,
detection, and determination of biomolecules such as proteins. In
this context, capillary gel electrophoresis (CGE) is an electrophoretic mode which uses a sieving matrix as buffer for the
separation of high molecular weights compounds [16]. CGE
presents the advantages of automation, on-column detection,
and higher resolution than the traditional SDS-PAGE. Thus, the
aim of this work was to use CGE as an alternative to SDS-PAGE for
the separation of olive proteins and to study the potential of
obtained protein profiles to differentiate olive varieties according
to their presumed geographical origin.