AgNPs were synthesized from AgNO3 by one step phyto bio-reduction procedure using WRA extract. Briefly, two sets of 100 mL of 1 mM AgNO3 solution in water in a 250 mL conical flask was added to 10 mL of WRA extract slowly drop by drop with continuous stirring at room temperature. One set was kept in darkness and another set was kept under direct sunlight at ambient temperature and pressure. The bio-reduction of AgNO3 to synthesize AgNPs in both the sets was monitored visually by the change in color of the reaction mixture with time. After synthesis, the reaction mixture containing AgNPs was centrifuged at 14,000 rpm for 30 min using a high speed centrifuge (Supra 22K, Hanil Science Industrial, Republic of Korea), after which the supernatant containing the water was discarded and the pellet was collected. The pellet was then washed twice in distilled water and dried to a powder using a vacuum dryer (LVS 201T, Ilmvac GmbH, Germany, UK)