The low yield of the biohydrogen production is the main constraint for its industrialization process. In order to improve its production,medium compositions of the hydrogen fermentation by Klebsiella pneumoniae ECU -15 were optimized through the response surface methodology (RSM).
Effect of the culture media optimization,pH and temperature on the biohydrogen production and the hydrogenase activities by Klebsiella pneumoniae ECU-15
Experimental results
showed that the optimum hydrogen production of 5363.8 ml/L was obtained when the concentration of
glucose, the ammonium sulfate and the trace elements were 35.62 g/L,2.78 g/L and 23.15 ml/L at temperature 37.0 C, pH 6.0.H2 evolving hydrogenase was greatly enhanced by the optimization of the medium
compositions.
The activity ofH2 evolving hydrogenase increased with the temperature,and decreased
with the pH,while the activity ofthe uptake hydrogenase increased with the temperature and the pH.
So the biohydrogen production process ofthe K. pneumoniae ECU-15 was the comprehensive results of
the evolution hydrogen process and the uptake hydrogen process.
The low yield of the biohydrogen production is the main constraint for its industrialization process. In order to improve its production,medium compositions of the hydrogen fermentation by Klebsiella pneumoniae ECU -15 were optimized through the response surface methodology (RSM).
Effect of the culture media optimization,pH and temperature on the biohydrogen production and the hydrogenase activities by Klebsiella pneumoniae ECU-15
Experimental results
showed that the optimum hydrogen production of 5363.8 ml/L was obtained when the concentration of
glucose, the ammonium sulfate and the trace elements were 35.62 g/L,2.78 g/L and 23.15 ml/L at temperature 37.0 C, pH 6.0.H2 evolving hydrogenase was greatly enhanced by the optimization of the medium
compositions.
The activity ofH2 evolving hydrogenase increased with the temperature,and decreased
with the pH,while the activity ofthe uptake hydrogenase increased with the temperature and the pH.
So the biohydrogen production process ofthe K. pneumoniae ECU-15 was the comprehensive results of
the evolution hydrogen process and the uptake hydrogen process.
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