The reaction contained 50 ng template DNA, 1X PCR buffer, 1.5 Mm MgCl2, 0.2 mM dNTP, 250 nM each of forward and reverse primers and 0.25U Taq polymerase in a total volume of 10 ll. PCR amplification was carried out in an M&J Thermal cycler. The three SSR marker (158, 169 and 22RY28) profiles