METHODOLOGY
Materials
The main source of sponges were collected from Bunaken Bay. Identification for the species of the active marine sponge was conducted in the Faculty of Biology, Gadjah Mada University. Based on its morphology, tissue and the specula, the most active sample (MD-02) was identified as Petrosia sp.. All the solvents used in this study are qualified as pro-analysis (E. Merck), otherwise stated. These include n-hexane, ether, acetone, chloroform, ethanol, methanol, ethyl acetate. TLC plates, silica gel GF 254. Spot detectors Dragendorf. Brine Shrimp Lethality Test (BST): Brine shrimp eggs from local fish shop, sea water, yeast.