FZB42 has been shown to reduce nematode eggs in roots,
juvenile worms in soil, and plant galls on tomato (BurkettCadena
et al., 2008). In order to identify specific-nematiciderelated
genes, a random transposon insertion library of FZB42
was screened for relevant genes involved in nematicidal activity
and – surprisingly – a gene within the pzn gene cluster was
identified as a pathogenic factor against nematodes. Further
experiments revealed that PZN displayed a moderate nematicidal
activity (Liu et al., 2013).
By transposon mutagenesis of the FZB42 mutant strain RS06,
which is deficient in Sfp-dependent synthesis of LP, polyketides,
and bacilysin, we identified a hitherto unknown gene cluster
involved in synthesis and posttranslational processing of a
novel circular bacteriocin, named amylocyclicin (Figure 3).
It became apparent that amylocyclicin inhibits growth of
bacterial strains closely related to FZB42 suggesting that this
bacteriocin might have a function in competing with other
Bacillus strains attracted to the plant rhizosphere
FZB42 has been shown to reduce nematode eggs in roots,juvenile worms in soil, and plant galls on tomato (BurkettCadenaet al., 2008). In order to identify specific-nematiciderelatedgenes, a random transposon insertion library of FZB42was screened for relevant genes involved in nematicidal activityand – surprisingly – a gene within the pzn gene cluster wasidentified as a pathogenic factor against nematodes. Furtherexperiments revealed that PZN displayed a moderate nematicidalactivity (Liu et al., 2013).By transposon mutagenesis of the FZB42 mutant strain RS06,which is deficient in Sfp-dependent synthesis of LP, polyketides,and bacilysin, we identified a hitherto unknown gene clusterinvolved in synthesis and posttranslational processing of anovel circular bacteriocin, named amylocyclicin (Figure 3).It became apparent that amylocyclicin inhibits growth ofbacterial strains closely related to FZB42 suggesting that thisbacteriocin might have a function in competing with otherBacillus strains attracted to the plant rhizosphere
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