The results of the present study provide a foundation for pathogenicity tests in future
research. The cassava leaves were very delicate and often rot after five days under in vitro conditions; therefore, the leaves on plants are suitable materials for the test. By inoculating leaves with conidial solution, Ahmed et al. (2009) found that the spots appeared at the ninth day of culture, and the characteristic lesions with darker peripheries formed by the 13th day. In the present study, lesions did not form with mycelia, and a similar result was obtained using conidial solution.