In this study using enrichment technique biodegradation of organophosphorous pesticide, (Chloropyrifos and
Ethion) was performed. Two fungal isolates Trichoderma harznaium and Rhizopus nodosus isolated from the
contaminated soil by enrichment technique, was capable of metabolizing the organophosphorous insecticide
(Chloropyriofos and Ethion) as a sole carbon source when grown in burkes mineral medium at concentration of
100 PPM respectively. Fungi were able to degrade 70-80% of the parental molecule (technical grade) in 21 days
period of incubation. Further, efficiency of degradation was increased by 10-20% with the supplementation of
0.1% dextrose to the mineral media. Desertion of Chloropyrifos, Ethion and its metabolites were analyzed by thin
layer chromatography and gas Chromatography. It was evident from the result that the isolated fungi could be used
to bioremediate the area contaminated with organophosphorous pesticide.