3.1. Screening for bacterial antagonist producing volatile antimicrobial
Two hundred and six strains of bacteria were isolated from the
soil using repeated plate streaking (Fig. 1). Preliminary screening
showed that 4 isolates had readily observed inhibitory activity towards
C. gloeosporioides mycelia growth, identified as TB09, TB30,
TB52 and TB72. The diameters of mycelia growth were inhibited
over 80% by TB09 and TB72 and reduced over 30% in the presence
of volatiles released from TB30 or TB52 (Table 1).