2.6. Ascorbate oxidase-containing tissues
The same authors (Sezgintürk and Dinçkaya, 2004) developed a biosensor for reduced glutathione (GSH) using cucumber (Cucumis sativus) tissue homogenate. Glutathione protects cells from free radicals and reactive oxygen species and is also involved in aminoacid transport, protein and DNA synthesis, etc. Cucumber tissue contains high levels of ascorbate oxidase, a member of the family of multicopper blue oxidases, which catalyses the oxidation of ascorbic acid. Glutathione inhibits this enzyme, changing the oxygen level. The limit of detection of the biosensor was 0.1 μM, and its application to
real samples of potato, broccoli and tomato provided results comparable to those obtained by Ellman's method. In addition to this, biosensors were functional even after 2 months, when usually GSH biosensors have a lifetime of 10 days.
2.6. Ascorbate oxidase-containing tissuesThe same authors (Sezgintürk and Dinçkaya, 2004) developed a biosensor for reduced glutathione (GSH) using cucumber (Cucumis sativus) tissue homogenate. Glutathione protects cells from free radicals and reactive oxygen species and is also involved in aminoacid transport, protein and DNA synthesis, etc. Cucumber tissue contains high levels of ascorbate oxidase, a member of the family of multicopper blue oxidases, which catalyses the oxidation of ascorbic acid. Glutathione inhibits this enzyme, changing the oxygen level. The limit of detection of the biosensor was 0.1 μM, and its application toreal samples of potato, broccoli and tomato provided results comparable to those obtained by Ellman's method. In addition to this, biosensors were functional even after 2 months, when usually GSH biosensors have a lifetime of 10 days.
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