The hindquarter muscle samples from dead transgenic
positive and negative calves were used for fatty acid
analysis according to the procedures from Kang and
Wang (2005). Briefly, tissue homogenate from muscle
was mixed with 1 mL hexane and 1 mL 14 % BF3/
MeOH reagent in a glassmethylation tube and evaporated
with nitrogen. Then, the mixtures were incubated in a
boiling water bath for 1 h. After cooled down to room
temperature, the mixtures were blended with 1 mL H2O
and centrifuged for 1 min. And then the upper hexane
layer was reserved and concentrated under nitrogen.
Fatty acid methyl esters were analyzed by gas chromatography
(Agilent 7890A). The peak area was calculated
by the peak height and width. Identification of
components was determined by comparison of retention
times with those of authentic standards (Sigma).