Since flavanones are relatively non-labile compared to vitamin
C and carotenoids, robust analytical methods can be employed
for extraction of flavanones[3,9,10]. The problems during extraction are better understood when real samples are used rather than
model standard matrices[11]. Solubility and mass transfer of the
analytes of interest not only depend on physicochemical properties of the compounds themselves but also greatly influenced by
the other non-specific analytes in the matrix[12]. Therefore, these
aspects require a detailed investigation in fruits due to their complex matrices. Traditionally, flavanones have been extracted with
aqueous solvents from freeze-dried samples to increase the tissue permeability to the solvent[5]. However, addition of water is
not required when extracted directly from fruit juice[13]. Maceration or blending, centrifugation and filtration are three commonly
followed extraction steps for citrus flavanones [13].