The generation of cell suspension and the subsequent casting of the plugs should be performed as rapidly as possible in order to minimize premature cell lysis. If large numbers of samples are being prepared, it is recommended that they be processed in batches of around 10 samples at a time. Once the first batch of isolates are in the cell lysis incubation, then start preparing the cells suspensions the next group samples, and so on. All batches can be lysed and washed together,
since additional lysis time will not affect the initial batches.