An efficient and reproducible procedure for thelarge-scale propagation of Dendrocalamus asper is described.direct shoot
proliferation was induced in aseptic inter node cultures of D. asper on modified Murashige and Skoog’s (1962) medium
supplemented with 0.5mg/l benzyladenine (BA). Multiple shoots (1–25) were formed within 4 weeks of internode culture without
root formation. The shoot-forming capacity of inter node was influenced by the BA concentration in the medium. Proliferating
shoot cultures were established by repeatedly subculturing shoots in propagules of 3 shoots each. A multiplication rate of 15–16
fold was achieved on MS medium +2.0 mg/l BA. Roots were formed on excised propagules. Callus formed when inter node
cultured on medium containing .5 kin +3.0 mg/l 1-naphthaleneacetic acid (NAA). Plantlets were hardened, acclimatized and
established in soil, where they exhibited normal growth.