Briefly,0.2 ml sample extract (1 mg/ml) was mixed with 1 ml of 10-fold dilution of Folin–Ciocalteu reagent and 0.8 ml of 15% (w/v) Sodium bicarbonate solution and kept at room temperature for 30 min.
Briefly,0.2 ml sample extract (1 mg/ml) was mixed with 1 ml of 10-folddilution of Folin–Ciocalteu reagent and 0.8 ml of 15% (w/v) Sodium bicarbonate solution and kept at room temperature for 30 min.