Acetic acid bacteria are a large group of obligate aerobic Gram-negative bacteria which are
commonly found in association with various kinds of sugary material. They are divided into four groups,
namely Acetobacter, Acidomonas, Frateuria and Gluconobacter, these being slightly different in some
physiological characteristics [3]. The two most studied acetic acid bacteria are Acetobacter and
Gluconobacter due to their economical importance. Acetic acid bacteria are found in association with
honey and honey bees [4-6]. However, to date, apparently no studies attempting to isolate these
bacteria from Thai honey have been reported. In this study, the selective enrichment procedure for the
isolation of acetic acid bacteria of particular thermotolerant strains from honey was carried out.